Determination of Amylum Amylose Content by Amylose Analyzer

The seeds of the Fagaceae are called acorns and there are more than 300 species in our country. The acorn seed is rich in nutrients, among which the crude starch content is as high as 50% to 60%, soluble sugar 2% to 8%, protein 3% to 6%, crude fiber 3% to 5%, crude fat 1 % to 5%, tannins 5.03% to 11.79%. The starch extracted has a wide range of applications and can be used as sizing, soil and cloth printing and dyeing. What is the composition of amylose and amylopectin extracted from acorn starch? This requires an accurate detection and analysis by an accurate amylose analyzer to make the final conclusion.

The dry acorn shelling, weighing a certain amount of rubber added to 3 to 5 times the water, soak at room temperature for 18 to 24h, until the soaking water without bitterness. Grind, over 100 mesh sieve, static settlement 6 ~ 8h. Remove the upper layer of liquid, shovel the dark gray slurry above, sieve the lower layer of starch over 200 mesh, then soak it twice with 0.2% NaOH for 8 hours. After discarding the soaking solution, add 4% to 6% of 30%. H2O2 bleaching, pH 10 ~ 11, 45 ° C stirring reaction 4 ~ 6h. After clarification, discard the supernatant liquid, and then neutralized with 2% HCl to pH 5 ~ 6, clarified, remove the supernatant, at 45 °C Dry, crushed 100 mesh sieve, spare.

In the preparation of acorn starch, the pulp is refined, sieved and allowed to stand. After standing, the main component of the upper liquid is tannin, followed by soluble sugar, polysaccharide, protein and pectin; there is a thin layer of brown or dark gray pulp in the middle. The bottom layer is starch. When determining the amylose content of acorn starch, there are large differences in the results obtained by different methods, such as the amylose content measured by the Juliano colorimetric method. Measured at 2% to 4% higher; using the colorimetric method, select different amylose standard products, the measured results are also quite different, this is due to different sources of amylose molecular size, spiral The number, structure, etc. are different.

The analysis by amylose analyzer showed that the amylopectin was branched in the treatment solution and there was a large steric hindrance. This property could be used to separate amylose and amylopectin, Multiple crystallization extractions can increase the purity of amylose to more than 95%.

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